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a break at another point forms the linear integrative prophage (see g. 14.18). 19. The prophage region of the Hfr chromosome enters the F cell with no repressor present. The situation is thus similar to regular phage infection, which can go either way (lysogenic or lytic cycles). 21. The cells that form colonies do not contain a prophage. The initial heat shock inactivated the repressor and allowed the prophage to excise. The lower temperature activated the repressor, and the repressor bound to the excised phage DNA, preventing gene expression and reintegration of some of the phages. These cytoplasmic phages failed to replicate and were lost during cell division. The cells that retained the virus were lysed when the temperature was raised because the phage could now make RNA. barcode generator excel download How Excel creates barcodes | PCWorld
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